Browsing by Author "Lateef Esther Oluwafunmilade"

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    Comparative Microbiological and Physicochemical analysis of Domestic and Industrial wastewater in Ado-Odo Ota LGA, Ogun State, Nigeria
    (Covenant Journal of Health and Life Science, 2023) Banjo Temitope Temitayo; Lateef Esther Oluwafunmilade; Aina Y.O.; Lateef S.A.; Agboola D. A.
    Wastewater from industrial and domestic sources flows into major bodies of water where people drink and bathe, resulting in a high incidence of severe water- borne infections. This study aims to compare the microbiological and physicochemical parameters of domestic and industrial wastewater in Ado-Odo LGA, Ogun State, Nigeria. Microorganisms were isolated from collected wastewater samples and were phenotypically identified using standard microbiological methods. Antimicrobial Sensitivity testing was carried out using Kirby Bauer`s disk diffusion technique. The physicochemical analysis of the water samples was also determined using standard analytical methods. A total of nineteen microorganisms were isolated from the samples collected with the predominant organism isolated from the two sources being Escherichia coli. The microorganisms isolated from the industrial wastewater sample were Citrobacter freundii (37.5%), Escherichia coli (37.5%), and Proteus vulgaris (25.0%) while those isolated from the domestic wastewater sample were Klebsiella oxytoca (18.18%), Escherichia coli (45.45%), Proteus vulgaris (9.09%), Staphylococcus saprophyticus (9.09%) and Staphylococcus aureus (18.18%). The physicochemical analysis conducted indicated that there were lower levels of impurities in industrial wastewater pH (4.1 ± 0.14), Electrical conductivity (479 ± 1.41), Total Dissolved Solids (223.5 ± 3.53), and Chloride content (168.12) compared to the domestic wastewater with pH (8.05 ± 0.07), Electrical Conductivity (1928± 4.24), Total Dissolved Solids (765.5 ± 0.70) and Chloride content (238.224) which suggests that industrial wastewater has been subjected to series of chemical and biological processes. The presence of Escherichia coli in higher levels in domestic wastewater indicates fecal contamination which is a major public health challenge since this wastewater leaks into various water bodies.
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    Comparative studies on ascorbic acid production by single and mixed cultures of Aspergillus flavus and A. tamarii
    (Covenant Journal of Health and Life Science, 2023) Banjo Temitope Temitayo; Akinduti Akinniyi Paul; Lateef Esther Oluwafunmilade
    Ascorbic acid is an antioxidant which promotes increased resistance to infection and acts as preservatives in foods. The complexity and expensive nature of its production led to the search for a relatively simple and cheap method of ascorbic acid production. Hence, this study investigated the potentials of mixed cultures of Aspergillus flavus and A. tamarii for increased ascorbic acid production. Optimization of the fermentation process was carried out at pH (4 – 8), temperature (30 - 45 °C) and agitation speed (60 - 160 rpm) for 96 h. Titration and High Performance Liquid Chromatography techniques were used to determine the quantity of ascorbic acid produced. The highest quantities of ascorbic acid were produced at pH (5.0), temperature (40 °C) and agitation speed of 100 rpm at 96h of fermentation. The highest quantity of ascorbic acid (9.648 g/L) was produced by the mixed culture compared to acid yield of 6.248 and 7.246 g/L by A. flavus and A. tamarii respectively. The ability of the mixed cultures of A. flavus and A. tamarii in hyper-production of ascorbic acid is therefore established in this study.
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    GC MS analysis of the Phytocomponents in the plant extracts of Bitter Apple ( Citrullus Colocynthis ) and their Antibacterial activities against selected pathogenic and food spoilage microorganisms
    (Covenant Journal of Physical and Life Sciences, 2022) Banjo Temitope Temitayo; Lateef Esther Oluwafunmilade; Malomo M.O.; Chuba C.T.
    The antibacterial potency of the ethanolic and aqueous extracts of the seeds and fruit epicarp of Citrullus colocynthis was evaluated against Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus using agar-well diffusion method. GC-MS analysis and phytochemical screening of the ethanolic and aqueous extracts of the seeds and fruit epicarp were carried out. The ethanolic and aqueous extracts of the fruits of the bitter apple showed inhibitory activities against the test organisms with zones of inhibition ranging between 2 mm and 16 mm, However, the range of inhibition with the seed extract was 5 mm and 18 mm. The GC-MS analysis revealed a total of forty-four and forty-nine phyto-compounds in the extracts of fruits and seeds of C. colocynthis respectively. This study showed that ethanolic extracts of the seeds of bitter apple could be employed in the pharmaceutical industry as a potent antimicrobial agent in the formulation of drugs.
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    Identification and Evaluation of Fungal Pathogens in Pond-Cultivated Catfish from Ketu Adie-Owe, Ado Odo Local Government Area, Ogun State (Nigeria)
    (Journal of Analytical Sciences and Applied Biotechnology, 2023) Lateef Esther Oluwafunmilade; Dunkwu Tobechukwu Christopher; Olawale Solomon Kolapo
    Fish remains a crucial component of human nutrition, providing essential high protein and mineral content for survival. This study, conducted in Ketu Adie-Owe, Ado Odo Local Government Area of Ogun State, Nigeria, aims to isolate and comprehend fungal pathogens in pond catfish ecosystems. Three male Clarias gariepinus fish samples were collected from a concrete fish pond in Apena Village, Ketu, Igbesa, and transported aseptically to the Microbiology Laboratory at Crawford University for microbiological analysis. Sabouraud Dextrose Agar with chloramphenicol, to inhibit bacterial growth, was prepared for the isolation of fungi following the manufacturer’s instructions. Skin and intestine samples from each fish were processed, leading to the identification of various fungal species, including yeast and mold types. Notable isolates included Trichosporon spp., Malassezia (yeasts), Fusarium oxysporum, and Arthroderma quadrifidum (molds), each potentially contributing uniquely to the pondwater ecosystem. Understanding these isolates is important due to their potential to harm fish health, reduce marketability, and pose health risks to humans and animals. The study raised concerns about possible water contamination in the catfish pond, highlighting the risk of fish infections under such conditions. The research also underscored the ecological importance of fungi in pond catfish ecosystems, particularly in nutrient cycling and organic matter decomposition. The presence of pathogenic fungi emphasizes the need for vigilant monitoring and management to protect fish health, aquaculture productivity, and public well-being