Non-plasmid mediated multi-drug resistance in Vibrio and Aeromanas spp. isolated from seafoods in Lagos.
Date
2010
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Internet Journal of Food Safety
Abstract
Fifty seafood samples comprising of shrimps, crabs and cuttlefish collected from fishing companies in Lagos between September 2007 and May 2008, were cultured on TCBS agar after enrichment in APW. Using API 20E system and complementary biochemical test, 83 VIbrio species and 3 Aeromonas hydrophila were identified from the samples. The Vibrio species encountered comprised of V. cholerae (3) V. parahaemolyticus (5) V. mimicus (16) V. alginolyticus (37) and V. harveyi (12), V. vulnificus (10) and Aeromonas hydrophila (3).
Antimicrobial susceptibility carried out using standard procedures revealed that all the 86(100%) isolates were resistant to,Augmentin, Chloramphenicol and Amoxicillin (Amx-Au -Chl). Ten(11.6%) of the isolates had multiple resistance to all the 10 antibiotics tested: Gentamycin, Nitrofuratoin, Tetracycline, Chloramphenicol, Amoxicillin, Ofloxacin, Cotrimoxazole, Augmentin, Ciprofloxacin, Ceftriazone (Amx-Aug-Chl-Nit-Cip-Tet-Ofl-Gen-Cot-Cro).Plasmid characterization revealed that only four (4.7%) of the Eighty-six isolates haboured plasmid DNA. These were two strains of V. alginolyticus with plasmids of molecular weight of 25.0 Kbp and 9.416 Kbp each and two strains of V. mimicus with plasmids of molecular weight 4.361 Kbp each.None of the Aeromonas specie haboured plasmids. This shows that antibiotic resistance in Vibrio and Aeromonas species in this environment is not plasmid mediated.
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Agbaje-Daniels F. et al. (2010). Non-plasmid mediated multi-drug resistance in Vibrio and Aeromanas spp. isolated from seafoods in Lagos. Internet Journal of Food Safety. 12; 10-15.