Browsing by Author "Adeleye Isaac Adeyemi"

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    Antimicrobial Activity of Essential Oil and Extracts of Gongronema latifolium Decne on Bacterial Isolates from Blood Stream of HIV Infected Patients
    (Journal of Pharmacology and Toxicology, 2011) Agbaje-Daniels Folashade; Adeleye Isaac Adeyemi; Omadime M.
    The essential oil as well as aqueous and ethanolic extracts of Gongronema latifolium leaves was evaluated for antimicrobial activity against bacteria isolated from blood streams of HIV patients in Lagos. Using agar diffusion method, the essential oil and the extracts showed moderate inhibitory activity against all the Stphylococcus sp., Escherichia coli, Shigella sp., Salmonella sp., Klebsiella pneumonia, Pseudomonas aeruginosa, Onchrobactrum anthropi and Candida albicans. The zones of inhibition values recorded were comparable to control antibiotic ampicillin but less than that of Ciprofloxacin and Chloramphenicol. The MIC for essential oil ranged between 5-40 μg mL-1, while MBC also ranged between 5-40 μg mL-1, the MIC and MBC for ethanol extract ranged between 3.125-12.5 mg mL-1 and 3.125-25.0 mg mL-1, while aqueous extract MIC range between 6.25-25.0 mg mL-1 and MBC also ranged between 6.25-25.0 mg mL-1, respectively. Extracts of Gongronema latifolium may be useful in ethnomedicine and in the treatment of blood stream infections in HIV patients. Essential oil from Gongronema latifolium leaves (Endl.) Decne was obtained by hydrodistillation and analyzed using Gas Chromatography/Mass Spectrophotometry (GC-MS). The oil was dominated by linear aliphatic compounds (27.06%),unsaturated fatty acids which was characterized by high percentage of Phthalic acid (18.61%), oleic acids (5.2%), arachidic acid (2.34%) and fumaric acid (2.22%). Monoterpenes including camphor, β-Cymene and phytol.
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    Characterization And Pathogenicity Of Vibrio Spp. Contaminating Seafoods In Lagos, Nigeria.
    (Internet Journal of Food Safety,, 2010) Agbaje-Daniels Folashade; Adeleye Isaac Adeyemi; Enyinnia V. A
    Twenty-five seafood samples (shrimps, crabs and cuttle fish) collected from seven Fishing Companies and some local fishermen in Lagos, Nigeria, were examined for the presence of Vibrio species. A total of 44 vibrios belonging to five different species were isolated. The most predominant species was Vibrio alginolyticus (31.8%), followed by V. harveyi (27.3%), V. mimicus (22.7%), V. parahaemolyticus (11.4) and V. cholerae (6.8%). All the strains of V. mimicus, V. parahaemolyticus and V. cholerae and some strains of V. alginolyticus and V. harveyi lysed human red blood cells. None of the isolates tested elicited fluid accumulation in the experimental mice. However, histology sections showed that V. parahaemolyticus, V. cholerae, two strains of V. mimicus, one strain each of V. alginolyticus and V. harveyi caused erosion of the epithelial linings of the intestines of the experimental mice. This indicates that the organisms can cause infections in humans by invasion of the epithelial linings of the intestine.
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    Characterization of Volatile Components of Epa-Ijebu: A Native Wonder Cure Recipe
    (Journal of Pharmacology and Toxicology, 2011) Agbaje-Daniels Folashade; Adeleye Isaac Adeyemi; Omadime M.
    Essential oils and volatile compound from Epa-Ijebu, a native cure-all concoction was extracted by hydrodistillation and analyzed using Gas Chromatography/ Mass Spectrometry (GC-MS) in an attempt to determine the major constituents that could be responsible for the acclaimed curative ability of this concoction over diverse illnesses. Twelve major organic compounds were identified of which Fatty acids were most prevalent (35.52%). This was followed by normal alkanes such as nonadecene, hexadecane, heptadecane, octadecane and heneicosane (constituting 26.5%),2-p-nitrophenyl-oxadiazol-1,3,4-one-5 (18.18%), Quinoline (5.96%), Benzothiazole (4.87%) , Alcohol (2.48%) and other compounds in traces. The prevalence of fatty acid extracts as well as quinoline, benzothiazole and alchohol may be responsible for the anti-bacterial and antifungal as well as other curative ability of this local concoction.
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    Non-plasmid mediated multi-drug resistance in Vibrio and Aeromanas spp. isolated from seafoods in Lagos.
    (Internet Journal of Food Safety, 2010) Agbaje-Daniels Folashade; Adeleye Isaac Adeyemi; Nwanze R.O.; Eyinnia V.A.; Smith S.I.; Fowora M.A.; Goodluck H.A.
    Fifty seafood samples comprising of shrimps, crabs and cuttlefish collected from fishing companies in Lagos between September 2007 and May 2008, were cultured on TCBS agar after enrichment in APW. Using API 20E system and complementary biochemical test, 83 VIbrio species and 3 Aeromonas hydrophila were identified from the samples. The Vibrio species encountered comprised of V. cholerae (3) V. parahaemolyticus (5) V. mimicus (16) V. alginolyticus (37) and V. harveyi (12), V. vulnificus (10) and Aeromonas hydrophila (3). Antimicrobial susceptibility carried out using standard procedures revealed that all the 86(100%) isolates were resistant to,Augmentin, Chloramphenicol and Amoxicillin (Amx-Au -Chl). Ten(11.6%) of the isolates had multiple resistance to all the 10 antibiotics tested: Gentamycin, Nitrofuratoin, Tetracycline, Chloramphenicol, Amoxicillin, Ofloxacin, Cotrimoxazole, Augmentin, Ciprofloxacin, Ceftriazone (Amx-Aug-Chl-Nit-Cip-Tet-Ofl-Gen-Cot-Cro).Plasmid characterization revealed that only four (4.7%) of the Eighty-six isolates haboured plasmid DNA. These were two strains of V. alginolyticus with plasmids of molecular weight of 25.0 Kbp and 9.416 Kbp each and two strains of V. mimicus with plasmids of molecular weight 4.361 Kbp each.None of the Aeromonas specie haboured plasmids. This shows that antibiotic resistance in Vibrio and Aeromonas species in this environment is not plasmid mediated. keywords: