Browsing by Author "Banjo Temitope Temitayo"
Now showing 1 - 15 of 15
Results Per Page
Sort Options
Item Antimicrobial, Antioxidant and Other Pharmacological Activities of Ocimum Species: Potential to Be Used as Food Preservatives and Functional Ingredients(Food Reviews International., 2021) Banjo Temitope Temitayo; Javad Sharifi-Rad; Adetunji Charles Oluwaseun; Olaniyan Olugbemi T.; Ojo Stephen Kayode; Micheal Olugbenga Samuel; Osahon Itohan Roli; Olaogun Oluwatoyin Nimota; Banjo Temitope Oluwabunmi; Juliana Bunmi Adetunji; Farukh Sharopov; Natália Cruz-Martins; María del Mar ContrerasOcimum plants are commonly used culinary herbs and fragrant ornamental plants. Beyond this feature, the present review firstly describes scientific studies on the antimicrobial and antioxidant activities of several Ocimum species. Secondly, the use of Ocimum plants as ingredients for food preservation, such as meat, fish and dairy products was covered. Thirdly, the phytochemical composition has been reviewed to reinforce further standardization to be Ocimum plants used as preservatives. Finally, other pharmacological properties (cardioprotective activity, anti-diabetic activity, hepato-renal protective activity, anticancer activity) are also reviewed. Overall, these plants can serve to promote food preservatives and functional ingredients, but there are still some challenges to be overcome as this review points out.Item Ascorbic Acid Production by Aspergillus flavus and Aspergillus tamarii; Kinetic and Thermodynamic Study(Springer, 2020) Banjo Temitope Temitayo; Kareem Sarafadeen Olateju; Adeogun Abideen IdowuThis study investigated the fermentation kinetics and thermodynamics of ascorbic acid production from Brewery Spent Grain (BSG) using Aspergillus flavus and Aspergillus tamarii. Ascorbic acid fermentation of A. flavus and A. tamarii was performed at a temperature of 30 °C, agitation speed of 100 rpm and pH 5.0 at 96 h of fermentation. The thermodynamics, kinetics of the growth parameters and ascorbic acid production were studied using Monod, Contois and Teisser models. Teisser model gave the best fit as it obtained the highest maximum specific growth rate (μmax) and correlation coefficient of 0.184 h−1 and 0.997, respectively, at 40 °C, pH 5.0 and 0.6 g of BSG. The result showed that Teisser model gave a better description of each growth parameter. Hence, the production of ascorbic acid by A. flavus and A. tamarii is growth-associated.Item Bioethanol Production from Bitter Yam (Dioscorea dumetorum) and Water Yam (Dioscorea alata) Peels(Nigerian Journal of Microbiology, 2019) Banjo Temitope Temitayo; Banjo Temitope Oluwabunmi; Ogbonna C.B; Eze O.I.Bioethanol which is an alternative source of fuel to fossil fuels can be produced from renewable crops. However, some of these renewable feedstocks are food competitive. Hence, this study therefore investigated the production of bioethanol from bitter yam and water yam peels. Strains of Aspergillus spp and Saccharomyces cerevisiae were obtained from the Microbiology laboratory of the University and characterized using morphological characteristics. The spores of Aspergillus tamarii and colonies of Saccharomyces cerevisiae were cultured in bitter yam and water yam medium using the bitter and water yam peels as substrates for ethanol production at substrate concentrations of 5 – 30 %, temperature range of 25 - 45 oC, agitation speed of 60-160 rev/min and pH range of 4.0 - 8.0. Optimum bioethanol yield of 13 % was obtained with bitter yam peels at substrate concentration of 20 %, temperature of 35 oC, agitation of 100 rev/min and pH of 7.0. Similarly, optimum bioethanol yield of 11 % was obtained with water yam peels at substrate concentration of 20 %, temperature of 35 oC, agitation of 100 rev/min and pH of 5.0. Bioethanol yield from bitter yam peels was observed to be higher than that of water yam peels at substrate concentration of 20 %, temperature of 35 oC, agitation of 100 rev/min and pH of 7.0. This study shows the potential of bitter yam and water yam peels as substrates for the biosynthesis of ethanol which can serve as alternative source of fuel.Item Biosynthesis of Ascorbic Acid by Aspergillus Flavus and Aspergillus Tamarii Immobilized in Afzelia Africana Matrix.(Food and Applied Bioscience Journal, 2018) Banjo Temitope Temitayo; Kareem Sarafadeen Olateju; Akinloye Oluseyi Adeboye; Popoola TemitopeA novel matrix for the immobilization of ascorbic acid produced by Aspergillus flavus and Aspergillus tamarii was reported. Spores of A. flavus and A. tamarii were immobilized on Afzelia africana matrix cross-linked with glutaraldehyde (2.5%) and the effects of Afzelia africana gel concentration (9–13%), spore load (100–500 mg/100 ml), bead size (2–7 mm) and bead number (2–10) on ascorbic acid yield were determined. The immobilized fungi were cultured in a liquid fermentation medium containing BSG (0.6% w/v) for ascorbic acid production for 144 h. The ascorbic acid produced was quantified titrimetrically. The statistical analysis of the effects of gel concentration, spore load and bead size on ascorbic acid production showed no significant difference at p>0.05. However, there was significant difference in the effect of bead number on ascorbic acid production at p<0.05. Ascorbic acid yield of 8.5 g/L and 7.5 g/L was produced by Aspergillus tamarii and Aspergillus flavus respectively using 9 beads at 96 h of fermentation. The immobilized Aspergillus tamarii and Aspergillus flavus retained activities of 72% and 70% respectively after five repeated cycle and also exhibited increased activities over the free cells. This study shows the potential of Afzelia africana as a novel matrix for enhanced ascorbic acid production.Item Clonal diversity and spatial dissemination of multi-antibiotics resistant Staphylococcus aureus pathotypes in Southwest Nigeria(Plos one, 2021) Banjo Temitope Temitayo; Akinduti Akinniyi Paul; Osiyemi Joshua Adekunle; Ejilude Oluwaseun; El-Ashker Maged; Adeyemi Adewale Gideon; Obafemi Yemisi Dorcas; Isibor Patrick OmoregieSpread of genetically diverse Staphylococcus aureus characterized with multi-antibiotic resistance and regulated by high level agr functionalities in several communities in southwest Nigeria was investigated and evaluated for infection control. Staphylococcus aureus pathotypes recovered from 256 cases including purulent pus from skin infections, soft tissue aspirates, wounds, otorrhea, eye, throat and endocervical infections were assayed for biofilm and antibiogram. Further genotyped with micro-array, mapped for geospatial distribution and evaluated for clonal diversity and functional accessory gene regulators (agr). Significant Staphylococci infection among the ages (OR:0.021, CI:0.545–1.914) and female gender with prevalence rate of MSSA (53.0%) and MRSA (1.5%) (OR:1.021, CI:0.374–1.785) were observed. More than 52.5% resistance rates to tetracycline and amoxicillin with significant median resistance were observed in all the infection cases (p = 0.001). Resistance rate of 78.8% at MIC50 32μg/ml and MIC90 128μg/ml to amoxicillin-clavulanate, and more than 40% resistance to ceftazidime, ciprofloxacin and tetracycline of MIC90 and MIC50 at 32 μg/ ml were observed. Strains with multi-antibiotic resistance index above 0.83, high beta lactamase and strong biofilm clustered into separate phylo-group. Heterogeneous t442 (wound and pus), t657 (wound), t091 (ear) and t657 (ear and wound) revealed high phylogenetic diversity. Only 4.6% pvl+ MSSA-CC1 agrI, pvl+ MSSA-CC5 (13.6%) and pvl+ MRSA-CC7 agrII (4.6%), expressed enterotoxin, leukocidins, proteases and resistance gene determinants. Livestock clonal types clustered with identified community-associated strains. Clonal dissemination of resistant pvl+ MSSA-CC1 and MRSA-CC5 encoding agr were predominant in several peri-urban communities where adequate geno-surveillance, population target antimicrobial stewardship, extensive community structured infection control programs are needed to prevent further focal dissemination.Item Comparative Microbiological and Physicochemical analysis of Domestic and Industrial wastewater in Ado-Odo Ota LGA, Ogun State, Nigeria(Covenant Journal of Health and Life Science, 2023) Banjo Temitope Temitayo; Lateef Esther Oluwafunmilade; Aina Y.O.; Lateef S.A.; Agboola D. A.Wastewater from industrial and domestic sources flows into major bodies of water where people drink and bathe, resulting in a high incidence of severe water- borne infections. This study aims to compare the microbiological and physicochemical parameters of domestic and industrial wastewater in Ado-Odo LGA, Ogun State, Nigeria. Microorganisms were isolated from collected wastewater samples and were phenotypically identified using standard microbiological methods. Antimicrobial Sensitivity testing was carried out using Kirby Bauer`s disk diffusion technique. The physicochemical analysis of the water samples was also determined using standard analytical methods. A total of nineteen microorganisms were isolated from the samples collected with the predominant organism isolated from the two sources being Escherichia coli. The microorganisms isolated from the industrial wastewater sample were Citrobacter freundii (37.5%), Escherichia coli (37.5%), and Proteus vulgaris (25.0%) while those isolated from the domestic wastewater sample were Klebsiella oxytoca (18.18%), Escherichia coli (45.45%), Proteus vulgaris (9.09%), Staphylococcus saprophyticus (9.09%) and Staphylococcus aureus (18.18%). The physicochemical analysis conducted indicated that there were lower levels of impurities in industrial wastewater pH (4.1 ± 0.14), Electrical conductivity (479 ± 1.41), Total Dissolved Solids (223.5 ± 3.53), and Chloride content (168.12) compared to the domestic wastewater with pH (8.05 ± 0.07), Electrical Conductivity (1928± 4.24), Total Dissolved Solids (765.5 ± 0.70) and Chloride content (238.224) which suggests that industrial wastewater has been subjected to series of chemical and biological processes. The presence of Escherichia coli in higher levels in domestic wastewater indicates fecal contamination which is a major public health challenge since this wastewater leaks into various water bodies.Item Comparative microbiological and physicochemical evaluation of different blends of tiger nut (Cyperus esculentus) and date (Phoenix dactylifera l.) beverages(Anchor University Journal of Science and Technology, 2024) Banjo Temitope Temitayo; Osumare MuibatBackground: The rising cost of animal milk has led to studies being carried out on the formulation of plant-based milk products which can compare favourably in terms of nutritional content and general acceptability to consumers. Objectives: Hence, the objective of this study was to evaluate and compare the microbiological and physicochemical properties of different blends of tiger nuts and date juice. Methods: Fresh tiger nuts and dates were processed to formulate five beverage blends in these ratios 100:00 (A), 30:70 (B), 50:50 (C), 70:30(D), and 0:100 (E). The physicochemical parameters (pH, moisture content, total soluble solids (TSS), total titratable acidity (TTA), and carbohydrates) of the tiger nut-date blends were determined. Results: The values for the physicochemical parameters obtained are as follows; pH (6.20-6.51), Moisture content (87.11-89.21), TTA (0.021-0.034), TSS (10.79-12.89). Carbohydrates (12.11-13.61). Total plate counts of the beverage ranged from 0.1 to 1.0 x 103 CFU/mL which is below the microbial limit of acceptance of 2.0×105 CFU mL–1. The microorganisms isolated from the beverage are Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. In addition, the 50:50 tiger nut-date beverage has the highest overall acceptance by the panelists compared to other blends. Conclusions: The tigernut-date beverage (50:50 tigernut-date blend) produced in this study could serve as an alternative to milk and milk products.Item Comparative Preservative Potential of Shea Butter and Coconut Oil on Tomato Fruits (Solanum lycopersicum)(African Scientist, 2023) Banjo Temitope Temitayo; Bankole-Ojo Olufunso SamuelTomato fruits are perishable. Hence several methods have been adopted in their preservation which have not been generally accepted due to their health implications. Therefore, the aim of this study is to investigate the potential of coconut oil and sheabutter in the preservation of tomato fruits. Tomato fruits were washed and treated with coconut oil and shea butter which was varied at different concentrations of 3, 6, 9, 12 and 15 % (w/v). The tomato fruit was placed in well-aerated baskets for 30 days during which organoleptic observation and microbial analysis were investigated. The GC-MS analysis of the coconut and shea butter samples was carried out. The shea butter displayed a higher mean preservation rate of 62 % compared to that of coconut oil with 25 %. The fungal isolates from the fruits included Aspergillus niger, Candida krusei, Fusarium oxysporum, Candida species, Aspergillus fumigatus, Penicillium notatum and Aspergillus terreus. The GC-MS analysis of the coating materials revealed the presence of some compounds that may be responsible for their antimicrobial properties. The study showed that shea butter exhibits the highest preservation rate of tomato fruits thus minimizing waste and economic losses to the farmers and the country in general.Item Comparative studies on ascorbic acid production by single and mixed cultures of Aspergillus flavus and A. tamarii(Covenant Journal of Health and Life Science, 2023) Banjo Temitope Temitayo; Akinduti Akinniyi Paul; Lateef Esther OluwafunmiladeAscorbic acid is an antioxidant which promotes increased resistance to infection and acts as preservatives in foods. The complexity and expensive nature of its production led to the search for a relatively simple and cheap method of ascorbic acid production. Hence, this study investigated the potentials of mixed cultures of Aspergillus flavus and A. tamarii for increased ascorbic acid production. Optimization of the fermentation process was carried out at pH (4 – 8), temperature (30 - 45 °C) and agitation speed (60 - 160 rpm) for 96 h. Titration and High Performance Liquid Chromatography techniques were used to determine the quantity of ascorbic acid produced. The highest quantities of ascorbic acid were produced at pH (5.0), temperature (40 °C) and agitation speed of 100 rpm at 96h of fermentation. The highest quantity of ascorbic acid (9.648 g/L) was produced by the mixed culture compared to acid yield of 6.248 and 7.246 g/L by A. flavus and A. tamarii respectively. The ability of the mixed cultures of A. flavus and A. tamarii in hyper-production of ascorbic acid is therefore established in this study.Item Effect of some preservative methods on the physicochemical and organoleptic properties of pineapple and watermelon fruit juices(Ukrainian Journal of Ecology, 2021) Banjo Temitope Temitayo; Ogunmade Taiwo Oludare; Banjo Temitope Oluwabunmi; Ogundeji Rebecca Omotoyosi; Idowu A.A.; Olaitan A.J.Fruit juices are important sources of nutrient and it contains several important therapeutic properties that may reduce the risk of various diseases. This study was based on the effect of different preservative methods on fruit juices (pineapple and watermelon) and determination of the best method. The fruit juice were preserved using sodium benzoate, lime, pasteurization and the combination of sodium benzoate and pasteurization. Twenty three bacteria and twelve fungi were isolated from the fruit juice. Bacteria isolates include; Salmonella spp, Campylobacter jejuni, Shigella spp and Escherichia coli. Fungi isolated from the pineapple juice include: Aspergillus flavus, Aspergillus niger, Ochrocosis gallopava, Geotrichum candidum and Ochrococus gallapava among others. The result obtained shows that the juice maintained colour, aroma and taste when both sodium benzoate and pasteurization were used to compare when no preservation was used. There is a noticeable increase in the microbial load of the juice when lime was used as the only preservatives at day 0 (4 × 10-5) compared to when sodium benzoate and pasteurization was used at day 0 (1 × 10-5). While combination of sodium benzoate and pasteurization was the best among all other preservative methods. The pH value of the juice was also checked and it was observed that pineapple juice had the lower pH when compared with that of water melon.Item GC MS analysis of the Phytocomponents in the plant extracts of Bitter Apple ( Citrullus Colocynthis ) and their Antibacterial activities against selected pathogenic and food spoilage microorganisms(Covenant Journal of Physical and Life Sciences, 2022) Banjo Temitope Temitayo; Lateef Esther Oluwafunmilade; Malomo M.O.; Chuba C.T.The antibacterial potency of the ethanolic and aqueous extracts of the seeds and fruit epicarp of Citrullus colocynthis was evaluated against Bacillus cereus, Pseudomonas aeruginosa and Staphylococcus aureus using agar-well diffusion method. GC-MS analysis and phytochemical screening of the ethanolic and aqueous extracts of the seeds and fruit epicarp were carried out. The ethanolic and aqueous extracts of the fruits of the bitter apple showed inhibitory activities against the test organisms with zones of inhibition ranging between 2 mm and 16 mm, However, the range of inhibition with the seed extract was 5 mm and 18 mm. The GC-MS analysis revealed a total of forty-four and forty-nine phyto-compounds in the extracts of fruits and seeds of C. colocynthis respectively. This study showed that ethanolic extracts of the seeds of bitter apple could be employed in the pharmaceutical industry as a potent antimicrobial agent in the formulation of drugs.Item Microbial Production of Ascorbic Acid from Brewery Spent Grain (BSG) by Aspergillus flavus and Aspergillus tamarii(Food and Applied Bioscience Journal, 2018) Banjo Temitope Temitayo; Kareem Sarafadeen Olateju; Akinloye Oluseyi AdeboyeThis study evaluated the use of Brewery Spent Grain (BSG) as a novel substrate for the production of ascorbic acid using Aspergillus flavus and Aspergillus tamarii. Spores of Aspergillus flavus and Aspergillus tamarii were cultured in a liquid fermentation medium containing BSG (0.6% w/v) for ascorbic acid production. The process was studied at pH 4–8, temperature range 30–45°C, agitation speed range 60–160 rpm for 168 h. Stability studies and the effects of Ethylenediaminetetraacetic Acid (0.5–4.0 g/ml) on ascorbic acid production were studied. Ascorbic acid produced was quantified by titration techniques and with High Performance Liquid Chromatography (HPLC). The statistical analysis of the effects of temperature and agitation speed on ascorbic acid production showed no significant difference at p<0.05. However, there was significant difference in the effect of pH on ascorbic acid production at p<0.05. Optimum ascorbic acid yields of 7.25 g/L and 6.25 g/L were produced by A. tamarii and A. flavus respectively at pH 5.0, temperature (40°C) and agitation speed of 100 rpm at 96 h of fermentation. High Performance Liquid Chromatography (HPLC) showed that 6.248 g/L and 7.246 g/L of ascorbic acid were produced by A. flavus and A. tamarii, respectively. Ascorbic acid production by A. flavus and A. tamarii was completely inhibited at 4 g/ml of EDTA. This study shows the potential of BSG as a novel substrate for ascorbic acid production.Item Optimization and production of ascorbic acid by fusant cell of Aspergillus flavus and Aspergillus tamarii(Journal of King Saud University – Science, 2019) Banjo Temitope Temitayo; Kareem Sarafadeen Olateju; Akinduti Akinniyi Paul; Temitope Popoola; Akinloye Oluseyi AdeboyeAscorbic acid is an essential vitamin for the optimal growth of most animals including humans. The complexity and expensive nature of ascorbic acid production necessitates the development of a relatively simple and cost-effective method for its production. Hence, this study explored the potential of a somatic hybrid (fusant cell) of Aspergillus flavus and Aspergillus tamarii for enhanced production of ascorbic acid. Optimization studies of the ascorbic acid fermentation processes were performed at pH range of 4–8, temperature range of 30–45 C and agitation speed range 60–160 rpm for 96 h of fermentation. Quantification of the ascorbic acid produced was done titrimetrically. There was significant difference (P < 0.05) in the ascorbic acid production of the fusant cell and the parental cells. The ascorbic acid produced by the somatic hybrid was 8.85 g/L compared to its parental strains (3.92 g/L and 4.57 g/L). However, pH, temperature and agitation speed had no significant effects (P > 0.05) on ascorbic acid production. The study revealed that maximal ascorbic acid production of 9.95 g/L was obtained at pH 5.0, temperature 40 C and agitation speed of 100 rpm at 96 h of fermentation. This study shows the potential of fusant cell for enhanced ascorbic acid production.Item Optimization of Biodiesel Production from Spent Cooking Oil by Fungal Lipase Using Response Surface Methodology(Nigerian Journal of Biotechnology, 2018) Banjo Temitope Temitayo; Kareem Sarafadeen Olateju; Adio Olayinka Quadri; Osho Michael Bamitale; Omeike Sunday OsaizuaThis study evaluated the potential of optimizing Spent Cooking Oil (SCO) transesterification for biodiesel production using Response Surface Methodology (RSM). Potential biodiesel yield from transesterification was optimized using a three-level four factor Response Surface Central Composite Design (RSCCD) with methanol oil ratio (1:1 to 3:1), temperature range 35-45 °C, agitation speed range 100-200 rpm and enzyme load 10-20%. Biodiesel properties including fatty acid methyl ester (FAME), Flash Point (FP), Pour Point (PP) and kinematic viscosity were compared with American (ASTM D6751) and European Union (EN 14214) standards. Biodiesel production was optimum at 3:1 methanol to oil ratio, temperature of 35 °C, agitation speed of 150 rpm and 20% enzyme load. 9-octadecanoic acid hydroxyl methyl ester (33.83%) was the prominent FAME produced, while the viscosity (6 mm2/s), density (893 kg/m3), FP (260 °C) and PP (- 0.5 °C) all met both American and European standards. This study showed that RSM is a viable methodology which could be used for optimization of biodiesel production from biological sources.Item Phytopharmacotherapeutic and Antimicrobial Attributes of Bitter Apple (Citrullus colocynthis) - A Review(Covenant Journal of Physical & Life Sciences, 2021) Banjo Temitope Temitayo; Aina Y. O.; Falade F. A.Emergence of drug-resistant microorganisms over the past few decades has resulted in the search for bioactive compounds potent against these microorganisms. Phytochemicals derived from plants have shown positive curative properties and can be extracted from any part of the plant such as the roots, stem or flower. Bitter apple (Citrullus colocynthis) has been used traditionally for the treatment of infectious diseases and this has stimulated pharmacological interest in the active ingredients responsible for its medicinal properties. The plant also possesses several biological and pharmacological activities including anti-inflammatory, antidiabetic, antimicrobial, anticancer, and antioxidant. This review discussed the phytochemicals present in C. colocynthis, their mode of action and their antimicrobial attributes.